Effects of Ouabain in Ehrlich Tumor Development in vitro and in vivo

Ouabain (OUA) is a cardiotonic steroid with an immunomodulatory and anti-inflammatory role in different experimental models. Currently, the potential antineoplastic effect of OUA has been studied, however, research needed to better understand during tumor development. Therefore, our aim was investigate effects on Ehrlich (ET) development vitro vivo. To evaluate cytotoxic ET cells were incubated concentrations 24h 48h results showed that only [1000 ?M] decreased number viability two analyzed times. study vivo, Swiss mice pretreated 0.56 mg/kg intraperitoneally (i.p.) for three consecutive days. develop solid form, one hour after last day pretreatment, inoculated subcutaneously into footpad animals monitored 13 ascitic 3 able reduce thickness weight paw, addition popliteal lymph node. In tumor, reduced neutrophils macrophages increased lymphocytes peritoneum. Thus, we demonstrated affects both Our suggest new perspective anti-inflammatory, possible anti-cancer ouabain brings concepts about pathophysiological this substance.